High-Performance Liquid Chromatographic Quantitative Determination of Amcinonide and Benzyl Alcohol in Pharmaceutical Preparations

This paper describes a simple, precise, and validated high-performance liquid chromatographic method for the simultaneous quantitative determination of amcinonide and benzyl alcohol in pharmaceutical preparations. The separation was achieved using a Zorbax reversed-phase column (5 m, 250x4.6mm) at room temperature with an isocratic mixture of deionized water and acetonitrile (70:30 v/v) at a flow rate of 1.5ml/min and detection at 254 nm. The limits of quantification for amcinonide and benzyl alcohol were found to be 4-30 g/ml and 80-600 g/ml, respectively. The validity of the method was evaluated in terms of linear regression analysis, precision, specificity, accuracy and ruggedness. The developed methodology is an economic, time-saving, straight forward and precise assay for the determination of amcinonide and benzyl alcohol in pharmaceutical preparations. It can be readily utilized for quality assurance and R&D laboratories of pharmaceutical and cosmetic industry.